Procedure

1. Check sample for the presence of C₁₆ and C₁₈ fatty acids by GLC.
2. If phospholipids are present (C₁₆ and C₁₈ fatty acids) the extract with CHCl₃:CH₃OH:H₂O 16:8:1
3. Verify the absence of phospholipds by TLC (CHCl₃:CH₃OH:H₂O:Triethylamine 30:15:2.5:0.1).

References

• D. Karibian, C. Deprun and M. Caroff, J. Bacteriol. 175 (1993) 2988-2993
• M. Caroff, A. Tacken and L. Szabo, Carbohydr. Res. 175 (1988) 273-282